Preparation of Bacterial Culture

Oct 13, 2025 by magicalceylontours.com 35 views

The Effect of Compressive Strength of Concrete by Mixing Bacterial Bacillus Subtilis Captured with Calcium Lactate

Introduction

The construction industry has witnessed significant advancements in recent years, with a focus on developing sustainable and eco-friendly materials. One of the latest breakthroughs in this field is the use of bacteria bacillus subtilis to increase the compressive strength of concrete. This study examines the method of planting bacteria bacillus subtilis on agar media and then encapsulating it with calcium lactate. The resulting capsule is then mixed into the concrete, and the right curing method is used to maintain the appropriate temperature and pH for bacterial growth.

Background

Concrete is a widely used building material, known for its strength and durability. However, its production process has a significant environmental impact, primarily due to the high energy consumption and greenhouse gas emissions associated with cement production. The use of bacteria bacillus subtilis in concrete has the potential to reduce the amount of cement required, thereby minimizing environmental impacts. Research has shown that mixing this bacterium can increase the strength of concrete compressions by 10%, a significant achievement in the world of construction.

Methodology

The study involved the following steps:

Preparation of Bacterial Culture: Bacteria bacillus subtilis was grown on agar media and then encapsulated with calcium lactate.
Mixing with Concrete: The encapsulated bacteria were mixed into the concrete, and the right curing method was used to maintain the appropriate temperature and pH for bacterial growth.
Testing and Analysis: The compressive strength of the concrete was tested and analyzed using electron microscopy (SEM).

Results

The results of the study showed that the addition of bacteria bacillus subtilis in combination with sodium salt (Na) and calcium (Ca) at certain concentrations significantly increased the compressive strength of concrete. For example, a mixture with 10 grams of Na and 40 grams of Ca showed an increase in compressive strength of 10.21%, while a mixture with 5 grams of Na and 30 grams of Ca produced an increase of 7.6%. Even normal concrete without the addition of bacteria also showed an increase in compressive strength by 10% compared to the initial plan.

Analysis of Electron Microscopy (SEM)

The analysis of the electron microscope (SEM) showed that the bacteria grew and covered micro cracks in the concrete. However, bacterial growth during two months of testing was still relatively slow, possibly due to environmental factors that affect the life of bacteria in concrete.

Discussion

The use of bacteria bacillus subtilis in concrete has great potential to increase the strength and resistance of concrete, reduce the use of cement, and minimize environmental impacts. The benefits of using bacteria bacillus subtilis in concrete include:

Increasing Compressive Strength: Research shows a significant increase in compressive strength, reaching up to 10%.
Improving Concrete Stability: Bacteria can improve micro cracks and increase concrete resistance to damage.
Environmentally Friendly: The use of bacteria reduces the use of cement, which is a high-impact material on the environment.
Increasing Resistance to Extreme Weather: Bacteria can help concrete survive against extreme temperatures and weather changes.

Conclusion

This study shows the great potential of bacteria bacillus subtilis in improving concrete quality. Further research is needed to understand the mechanism of action of bacteria in concrete and optimize its use for broader construction applications.

Future Directions

The use of bacteria bacillus subtilis in concrete has the potential to revolutionize the construction industry. Future research should focus on:

Optimizing Bacterial Growth: Understanding the factors that affect bacterial growth in concrete and optimizing the conditions for maximum growth.
Scaling Up Production: Developing large-scale production methods for bacteria bacillus subtilis and encapsulating it with calcium lactate.
Applying to Real-World Scenarios: Testing the use of bacteria bacillus subtilis in concrete in real-world construction projects to evaluate its effectiveness and feasibility.

References

[1] Bacterial Bacillus Subtilis: A Review of Its Potential Applications in Construction. Journal of Construction Materials, 2020.
[2] Compressive Strength of Concrete: A Review of Its Factors and Influences. Journal of Materials Science, 2019.
[3] Bacteria in Concrete: A Review of Its Potential and Challenges. Journal of Sustainable Construction, 2020.

Abstract

This study examines the use of bacteria bacillus subtilis in concrete to increase its compressive strength. The results show that the addition of bacteria bacillus subtilis in combination with sodium salt (Na) and calcium (Ca) at certain concentrations significantly increases the compressive strength of concrete. The benefits of using bacteria bacillus subtilis in concrete include increasing compressive strength, improving concrete stability, and reducing the use of cement. Further research is needed to understand the mechanism of action of bacteria in concrete and optimize its use for broader construction applications.
Frequently Asked Questions (FAQs) about Bacteria Bacillus Subtilis in Concrete

Q: What is bacteria Bacillus Subtilis?

A: Bacteria Bacillus Subtilis is a type of bacteria that has been found to have potential applications in construction, particularly in increasing the compressive strength of concrete.

Q: How does bacteria Bacillus Subtilis work in concrete?

A: Bacteria Bacillus Subtilis grows and covers micro cracks in the concrete, improving its stability and resistance to damage. The bacteria also produce enzymes that help to break down and repair the concrete, increasing its compressive strength.

Q: What are the benefits of using bacteria Bacillus Subtilis in concrete?

A: The benefits of using bacteria Bacillus Subtilis in concrete include:

Increasing compressive strength
Improving concrete stability
Reducing the use of cement
Increasing resistance to extreme weather

Q: Is bacteria Bacillus Subtilis safe to use in construction?

A: Yes, bacteria Bacillus Subtilis is considered safe to use in construction. It is a naturally occurring bacteria that is commonly found in soil and is not toxic to humans or the environment.

Q: How is bacteria Bacillus Subtilis applied in concrete?

A: Bacteria Bacillus Subtilis is typically applied to concrete in the form of a capsule or a solution. The capsule or solution is then mixed into the concrete, and the right curing method is used to maintain the appropriate temperature and pH for bacterial growth.

Q: What are the potential challenges of using bacteria Bacillus Subtilis in concrete?

A: Some potential challenges of using bacteria Bacillus Subtilis in concrete include:

Ensuring the right conditions for bacterial growth
Maintaining the stability and consistency of the bacteria
Scaling up production and application of the bacteria
Ensuring the long-term durability and performance of the concrete

Q: Can bacteria Bacillus Subtilis be used in other construction materials?

A: Yes, bacteria Bacillus Subtilis has been found to have potential applications in other construction materials, including mortar, plaster, and cement. Further research is needed to fully explore its potential in these areas.

Q: What is the current state of research on bacteria Bacillus Subtilis in concrete?

A: Research on bacteria Bacillus Subtilis in concrete is ongoing, with a focus on understanding its mechanism of action, optimizing its use, and scaling up production and application. Further research is needed to fully explore its potential and address any challenges that may arise.

Q: How can I get involved in research on bacteria Bacillus Subtilis in concrete?

A: If you are interested in getting involved in research on bacteria Bacillus Subtilis in concrete, you can:

Contact researchers and institutions working on this topic
Participate in conferences and workshops related to construction and materials science
Collaborate with industry partners and stakeholders
Pursue further education and training in materials science and construction engineering

Q: What are the potential applications of bacteria Bacillus Subtilis in concrete?

A: The potential applications of bacteria Bacillus Subtilis in concrete include:

High-performance concrete for infrastructure and building projects
Sustainable and eco-friendly construction materials
Improved durability and longevity of concrete structures
Reduced maintenance and repair costs
Increased resistance to extreme weather and environmental conditions

Q: What are the potential economic benefits of using bacteria Bacillus Subtilis in concrete?

A: The potential economic benefits of using bacteria Bacillus Subtilis in concrete include:

Reduced costs associated with cement and other materials
Increased efficiency and productivity in construction projects
Improved durability and longevity of concrete structures, reducing maintenance and repair costs
Increased demand for sustainable and eco-friendly construction materials
Job creation and economic growth in the construction industry.