Increase preventive measures

Oct 13, 2025 by magicalceylontours.com 51 views

Aedes aegypti Mosquito Resistance Analysis from Helvetia Timur Village on the Active Ingredients of Permethrin with the Bottle Bioassay Test Method in 2022

Introduction

Dengue hemorrhagic fever (DHF) is a significant health problem that affects communities worldwide, both as endemic and epidemic diseases. In Helvetia Timur Village, Medan Helvetia District, it is listed as one of the highest DHF cases, occupying three out of fifteen locations with the most incidence. The spread of this disease can be controlled by reducing the reproduction of vectors that cause them through the use of insecticides. Permethrin, as one type of pyrethroid insecticide, works quickly by damaging the nervous system in the body of the insect.

Background of the Study

The purpose of this study was to determine the status of Aedes Aegypti resistance originating from the East Helvetia Village on the active ingredients of Permethrin using the Bottle Bioassay Test method. This study uses a quasi-experimental design, with a total sample of 125 female aedes aedes mosquitoes, consisting of 25 mosquitoes in the control group and 100 mosquitoes in the test group. The Bottle Bioassay Test method is a widely used technique for testing the susceptibility of mosquitoes to insecticides.

Methodology

The results of the larvae survey in Helvetia Timur Village show that the House Index value is 18%, Container Index 17.8%, and Breteau Index 30%. Based on the density rate, the East Helvetia Village is included in the moderate risk category of DHF transmission. Free index larvae (ABJ) reached 82%, which indicates high larvae density. In testing mosquitoes originating from Helvetia Timur Village with active ingredients permethrin for 2 hours, the percentage of mortality is calculated. The test results with a dose of 15 g/bottle showed 55 mosquitoes dead during the diagnosis, while at a dose of 75 g/bottle there were 76 mosquitoes that died. In the control group, no death was detected.

Results

The results of this study indicate that Aedes aegypti from Helvetia Eastern Village shows resistance to the two doses of permethrin, which is a diagnostic dose of 15 g/bottle and a dose of 75 g/bottle which is five times higher. This finding suggests that Permethrin is now ineffective as an insecticide to eradicate Aedes aegypti mosquitoes in the area. This is a serious concern for efforts to control DHF vectors, and emphasize the importance of finding alternative control strategies that are more effective and environmentally friendly.

Discussion

The phenomenon of mosquito resistance to insecticides, including permethrin, is a global problem that requires more attention. This resistance can occur due to repeated and irregular use of insecticides, as well as lack of community understanding of the importance of disease prevention and control. In the context of the East Helvetia Village, preventive measures need to be increased, including counseling on a clean environment to reduce mosquito breeding sites. The use of new insecticides with different mechanisms can also be considered to replace that is ineffective.

Conclusion

The community is advised to be more proactive in maintaining the cleanliness of the surrounding environment, for example by collecting rainwater, replacing water in a shelter every week, and using natural ingredients that can repel mosquitoes. Counseling and cooperation between the government and the community in controlling DHF must be strengthened to ensure better health for all citizens.

Recommendations

Increase preventive measures: Preventive measures need to be increased, including counseling on a clean environment to reduce mosquito breeding sites.
Use new insecticides: The use of new insecticides with different mechanisms can also be considered to replace that is ineffective.
Community involvement: The community is advised to be more proactive in maintaining the cleanliness of the surrounding environment.
Counseling and cooperation: Counseling and cooperation between the government and the community in controlling DHF must be strengthened to ensure better health for all citizens.

Limitations of the Study

Sample size: The sample size of this study is relatively small, which may limit the generalizability of the results.
Methodology: The Bottle Bioassay Test method may not be the most effective method for testing the susceptibility of mosquitoes to insecticides.

Future Research Directions

Investigate the mechanisms of resistance: Further research is needed to investigate the mechanisms of resistance to permethrin in Aedes aegypti mosquitoes.
Develop new control strategies: New control strategies that are more effective and environmentally friendly need to be developed to replace the ineffective use of permethrin.
Community-based interventions: Community-based interventions need to be developed to increase the awareness and understanding of the importance of disease prevention and control among the community.
Aedes aegypti Mosquito Resistance Analysis from Helvetia Timur Village on the Active Ingredients of Permethrin with the Bottle Bioassay Test Method in 2022: Q&A

Q: What is the significance of this study?

A: This study is significant because it investigates the resistance of Aedes aegypti mosquitoes to permethrin, a commonly used insecticide, in Helvetia Timur Village. The results of this study can inform the development of more effective control strategies for dengue hemorrhagic fever (DHF) in the area.

Q: What is the Bottle Bioassay Test method?

A: The Bottle Bioassay Test method is a widely used technique for testing the susceptibility of mosquitoes to insecticides. In this study, the Bottle Bioassay Test method was used to test the susceptibility of Aedes aegypti mosquitoes to permethrin.

Q: What were the results of the study?

A: The results of the study showed that Aedes aegypti mosquitoes from Helvetia Timur Village were resistant to both doses of permethrin tested, which is a diagnostic dose of 15 g/bottle and a dose of 75 g/bottle which is five times higher.

Q: What are the implications of the study's findings?

A: The study's findings have significant implications for the control of DHF in Helvetia Timur Village. The resistance of Aedes aegypti mosquitoes to permethrin means that this insecticide is no longer effective in controlling the population of these mosquitoes, which are the primary vectors of DHF.

Q: What are some potential solutions to the problem of resistance to permethrin?

A: Some potential solutions to the problem of resistance to permethrin include:

Increasing preventive measures, such as counseling on a clean environment to reduce mosquito breeding sites
Using new insecticides with different mechanisms to replace permethrin
Developing community-based interventions to increase awareness and understanding of the importance of disease prevention and control among the community

Q: What are some limitations of the study?

A: Some limitations of the study include:

The sample size of the study was relatively small, which may limit the generalizability of the results
The Bottle Bioassay Test method may not be the most effective method for testing the susceptibility of mosquitoes to insecticides

Q: What are some future research directions?

A: Some future research directions include:

Investigating the mechanisms of resistance to permethrin in Aedes aegypti mosquitoes
Developing new control strategies that are more effective and environmentally friendly
Conducting community-based interventions to increase awareness and understanding of the importance of disease prevention and control among the community

Q: What are some recommendations for policymakers and public health officials?

A: Some recommendations for policymakers and public health officials include:

Increasing funding for research and development of new control strategies for DHF
Implementing community-based interventions to increase awareness and understanding of the importance of disease prevention and control among the community
Developing and implementing policies to reduce the use of permethrin and other insecticides that are no longer effective in controlling Aedes aegypti mosquitoes.

Q: What are some recommendations for the community?

A: Some recommendations for the community include:

Being more proactive in maintaining the cleanliness of the surrounding environment
Collecting rainwater and replacing water in shelters every week
Using natural ingredients that can repel mosquitoes
Participating in community-based interventions to increase awareness and understanding of the importance of disease prevention and control among the community.