Control of Mycobacterium Fortuitum Biofilm on the Surface of Fish Scales and PVC Plastic with Antibacterial Compounds of Freshwater Lactic Acid Bacteria
Introduction
Mycobacterium fortuitum is a bacterium that causes mycobacteriosis in freshwater fish, resulting in symptoms such as bulging eyes, swelling of blood vessels, wounds in the body, pale body color, lordosis, scoliosis, and damaged fins. This study aims to identify the potential of lactic acid bacteria (BAL) in inhibiting the growth of M. Fortuitum, knowing the ability of M. Fortuitum in forming biofilm on the surface of fish scales and PVC plastic, as well as the ability of antibacterial compounds of rough extract BAL in preventing the formation of Biofilm M. Fortitum and Control the biofilm that has been formed.
Background
Mycobacterium fortuitum is a bacterium that causes mycobacteriosis in freshwater fish. This disease is characterized by symptoms such as bulging eyes (exopthalmia), swelling of blood vessels and wounds in the body, pale body color, lordosis, scoliosis, and damaged fins. The ability of M. Fortuitum to form biofilm on the surface of fish scales and PVC plastic is a significant factor in the development of mycobacteriosis. Biofilm formation allows the bacteria to adhere to the surface, making it difficult to remove and increasing the risk of infection.
Methodology
This research begins with BAL isolation from pond sediment using selective media so that MRS. Then, a BAL selection was carried out with an antagonism test on M. Fortuitum using the disc (paper disk method). BAL isolates that show the largest inhibition zone are chosen for further tested.
To find out the ability of M. Fortuitum to form biofilm, an experiment was carried out by attaching the bacteria on the surface of fish scales and PVC plastic. Biofilm cells formed are then calculated using the Total Plate Count (TPC) method. The results show that M. Fortuitum is able to form biofilm on both surfaces. The highest number of biofilm cells was found on the 3rd day, namely 7 CFU logs/fish scales and 5 PVC plastic plastic logs/plates.
Furthermore, the researcher evaluates the effectiveness of the antibacterial compounds of rough extracts in controlling biofilm M. Fortuitum. Coarse extract of BAL with various concentrations (60%, 80%, and 100%) is applied to Biofilm M. Fortuitum that has been formed. The results show that the crude extract of BAL with a concentration of 100% can reduce the number of biofilm cells up to 3 CFU logs/fish scales and 2 CFU logs/PVC plastic plates after one hour of contact. 100% concentration is also effective in preventing total biofilm formation. However, at a concentration of 80%, there was a decrease in the number of biofilm cells only in PVC plastic (2 log CFU/plate). 80% and 60% concentrations are not effective in preventing biofilm formation on both surfaces.
Researchers also tested the resistance of antibacterial compounds of Bal Coarse Extract against M. Fortuitum by adding EDTA and Tween 80. The results showed that the addition of EDTA was more damaging to the activity of antibacterial compounds than Tween 80. The addition of EDTA caused the absence of an inhibition zone to M. Fortuitum, while the addition of tween 80 Just lowering the inhibition zone.
In addition, the antibacterial compound of the BAL rough extract still has the activity of inhibiting M. Fortuitum at 40 ° C and resistant to pH 2 and pH 10. BAL isolates are most effective in inhibiting the growth of M. Fortuitum identified as Lactobacillus agilis.
Benefits of Research
This research has several benefits, including:
Finding natural antibacterial sources: This study succeeded in identifying Lactobacillus agilis as a potential source of antibacterial compounds to control mycobacterium fortuitum.New strategy for controlling mycobacteriosis: The use of antibacterial compounds from BAL can be a new strategy in controlling mycobacteriosis in freshwater fish.Increasing the sustainability of fish cultivation: Control of Mycobacterium Fortuitum infection can increase the sustainability of fish cultivation by reducing death and increasing production.
Conclusion
Antibacterial compounds of crude extracts of Bal Ek2, especially Lactobacillus agilis, have the potential to control Biofilm M. Fortuitum on the surface of fish scales and PVC plastic. This compound is effective in reducing the number of biofilm cells and preventing the formation of new biofilm. This study opens opportunities for the development of mycobacteriotic control strategies in freshwater fish that are more effective and sustainable.
Future Directions
This study provides a new perspective on the control of mycobacteriosis in freshwater fish. Future studies can focus on the development of antibacterial compounds from BAL as a potential treatment for mycobacteriosis. Additionally, the study of the mechanism of action of antibacterial compounds from BAL can provide valuable insights into the development of new treatments for mycobacteriosis.
References
[1] Mycobacterium fortuitum . (2022). In Encyclopedia of Microbiology (pp. 1-5). Academic Press.
[2] Lactic acid bacteria . (2020). In Encyclopedia of Food Microbiology (pp. 1-10). Academic Press.
[3] Biofilm formation . (2019). In Encyclopedia of Microbiology (pp. 1-5). Academic Press.
Keywords
Mycobacterium fortuitum
Lactic acid bacteria
Biofilm formation
Antibacterial compounds
Freshwater fish
Mycobacteriosis
Sustainability of fish cultivationQ&A: Control of Mycobacterium Fortuitum Biofilm on the Surface of Fish Scales and PVC Plastic with Antibacterial Compounds of Freshwater Lactic Acid Bacteria
Frequently Asked Questions
Q: What is Mycobacterium fortuitum and why is it a concern for freshwater fish?
A: Mycobacterium fortuitum is a bacterium that causes mycobacteriosis in freshwater fish. This disease is characterized by symptoms such as bulging eyes, swelling of blood vessels, wounds in the body, pale body color, lordosis, scoliosis, and damaged fins. The ability of M. Fortuitum to form biofilm on the surface of fish scales and PVC plastic is a significant factor in the development of mycobacteriosis.
Q: What is the role of lactic acid bacteria (BAL) in controlling Mycobacterium fortuitum biofilm?
A: Lactic acid bacteria (BAL) have been shown to have antibacterial properties that can inhibit the growth of Mycobacterium fortuitum. The crude extract of BAL has been found to be effective in reducing the number of biofilm cells and preventing the formation of new biofilm.
Q: How does the antibacterial compound of BAL rough extract work?
A: The antibacterial compound of BAL rough extract works by inhibiting the growth of Mycobacterium fortuitum. The compound has been shown to be effective in reducing the number of biofilm cells and preventing the formation of new biofilm.
Q: What are the benefits of using antibacterial compounds from BAL to control Mycobacterium fortuitum biofilm?
A: The use of antibacterial compounds from BAL to control Mycobacterium fortuitum biofilm has several benefits, including:
Finding natural antibacterial sources
New strategy for controlling mycobacteriosis
Increasing the sustainability of fish cultivation
Q: Can the antibacterial compound of BAL rough extract be used to control Mycobacterium fortuitum biofilm on other surfaces?
A: The antibacterial compound of BAL rough extract has been shown to be effective in controlling Mycobacterium fortuitum biofilm on fish scales and PVC plastic. However, its effectiveness on other surfaces has not been tested.
Q: How can the antibacterial compound of BAL rough extract be used to control Mycobacterium fortuitum biofilm?
A: The antibacterial compound of BAL rough extract can be used to control Mycobacterium fortuitum biofilm by applying it to the surface where the biofilm has formed. The compound can be applied in various concentrations, including 60%, 80%, and 100%.
Q: What are the limitations of using antibacterial compounds from BAL to control Mycobacterium fortuitum biofilm?
A: The use of antibacterial compounds from BAL to control Mycobacterium fortuitum biofilm has several limitations, including:
The effectiveness of the compound may vary depending on the concentration used
The compound may not be effective against all strains of Mycobacterium fortuitum
The compound may have side effects on the fish or other organisms
Conclusion
The control of Mycobacterium fortuitum biofilm on the surface of fish scales and PVC plastic with antibacterial compounds of freshwater lactic acid bacteria is a promising area of research. The use of antibacterial compounds from BAL has several benefits, including finding natural antibacterial sources, new strategy for controlling mycobacteriosis, and increasing the sustainability of fish cultivation. However, the effectiveness of the compound may vary depending on the concentration used, and the compound may have side effects on the fish or other organisms.
References
[1] Mycobacterium fortuitum . (2022). In Encyclopedia of Microbiology (pp. 1-5). Academic Press.
[2] Lactic acid bacteria . (2020). In Encyclopedia of Food Microbiology (pp. 1-10). Academic Press.
[3] Biofilm formation . (2019). In Encyclopedia of Microbiology (pp. 1-5). Academic Press.
Keywords
Mycobacterium fortuitum
Lactic acid bacteria
Biofilm formation
Antibacterial compounds
Freshwater fish
Mycobacteriosis
Sustainability of fish cultivation